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Women in Medicine and Science at Upstate: Patricia M. Kane PhD

This guide provides information on female pioneers associated with Upstate and its predecessor institutions who left lasting impacts on medicine and science. Please check back regularly as we will continue to add influential women to this guide.

Patricia M. Kane PhD

Patricia M. Kane completed her undergraduate degree at St. Lawrence University and her MS (1984) and doctoral degrees in bio-organic chemistry (1987) at Cornell University. She was awarded a National Institute of Health postdoctoral fellowship (1988-1990) and then joined the faculty of The College of William and Mary as an assistant professor of chemistry. She was the recipient of the National Science Foundation Presidential Young Investigators Award in 1991, before joining the faculty of the Department of Biochemistry and Molecular Biology as an assistant professor in 1992. She was promoted to full professor in 2003 and became chair of the department of Biochemistry in 2009.

Dr. Kane’s research on the function of proton-ATPase and the control of cellular pH has been continuously funded by the NIH since 1994, including the most recent $1.1 million award for continuing the study of how V-ATPase, a type of enzyme, knows where and how to help cells regulate pH. Long-term, this work could help researchers better understand neurodegenerative diseases and those that affect the immune system. She has authored 54 peer-reviewed publications, many written with the large number of postdoctoral, graduate and undergraduate students she has mentored.

Dr. Kane has been recognized for her teaching with the Upstate Excellence in Teaching Award (1995), was elevated to the SUNY Distinguished Faculty rank (2016), and was three times honored with the Outstanding Teacher Award from the Upstate College of Graduate Studies. She is also the recipient of the Upstate President’s Award for Excellence in Research (2004) and the Award for Research and Scholarship from the Research Foundation of SUNY (2007).

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Bibliography

  1. P. M. Kane, J. Erickson, C. Fewtrell, B. Baird., and D. Holowka (1986), "Crosslinking of IgE-receptor complexes at the cell surface: Synthesis and characterization of a long bivalent hapten that is capable of triggering mast cells and rat basophilic leukemia cells", Molecular Immun. 23:783-790.
  2. J. Erickson, P. M. Kane, B. Goldstein, D. Holowka, and B. Baird (1986), "Crosslinking of IgE-receptor complexes at the cell surface: A fluorescence method for studing the binding of monovalent and bivalent haptens to IgE", Molecular Immun. 23: 769-781.
  3. P. M. Kane, D. Holowka and B. Baird (1988), "Crosslinking of IgE-receptor complexes by rigid bivalent antigens >200 A in length triggers cellular degranulation",  J. Cell Biol. 107: 969-980.
  4. B. Baird, J. Erickson, B. Goldstein, P. Kane, A. Menon, D. Robertson, and D. Holowka (1988), "Progress toward understanding the molecular details and consequences of IgE-receptor crosslinking", in Theoretical Immunology (Perelson, A., ed.), pp. 41-59, Addison-Wesley, Reading, MA.
  5. B. Baird, R.J. Shopes, V.T. Oi, J. Erickson, P. Kane, and D. Holowka (1989), "Structural interactions of IgE and its high affinity receptor on the cell surface", Int. Arch. Allergy Appl. Immun. 88: 23-28.
  6. J. H. Rothman, C. T. Yamashiro, C. K. Raymond, P. M. Kane, and T. H. Stevens (1989), "Acidification of the lysosome-like vacuole and the vacuolar H+-ATPase are deficient in two yeast mutants that fail to sort vacuolar proteins", J. Cell Biol.109: 93-100.
  7. J. H. Rothman, C. T. Yamashiro, P. M. Kane, and T. H. Stevens (1989), "Protein targeting to the yeast vacuole", Trends in Biochem. Sci. 14: 347-350.
  8. P. M. Kane, C. T. Yamashiro, J. H. Rothman, and T. H. Stevens (1989), "Protein sorting in yeast:  The role of the vacuolar proton-translocating ATPase",  J. Cell Sci. Suppl. 11:161-178. (Invited review)
  9. P. M. Kane, C.T. Yamashiro, and T.H. Stevens (1989), "Biochemical characterization of the yeast vacuolar H+-ATPase", J. Biol. Chem. 264: 19236-19244.
  10. P. M. Kane, D. Holowka and B. Baird (1990), "Characterization of model antigens composed of biotinylated haptens bound to avidin", Immun. Invest. 19:1-25.
  11. C.T. Yamashiro, P.M. Kane, D. F. Wolczyk, R. A. Preston, and T.H. Stevens (1990), "The role of vacuolar acidification in protein sorting and zymogen activation: A genetic analysis of the yeast vacuolar H+-ATPase", Mol. Cell Biol. 10:3737-3749.
  12. P. M. Kane, C. T. Yamashiro, D. F. Wolczyk, N. Neff, M. Goebl, and T. H. Stevens (1990), "Protein splicing converts the yeast TFP1  gene product to the 69 kD subunit of the vacuolar H+-ATPase", Science 250:651-657.
  13. P. M. Kane, M. C. Kuehn, I. Howald, and T.H. Stevens (1992), "Assembly and targeting of peripheral and integral membrane subunits of the yeast vacuolar H+-ATPase",  J. Biol. Chem.267:447-454.
  14. P.M. Kane and T.H. Stevens (1992), "Subunit composition, biosynthesis, and assembly of the yeast vacuolar proton-translocating ATPase", J. Bioenerg. Biomemb. 24:383-393.
  15. P.M. Kane (1992), "Biogenesis of the yeast vacuolar H+-ATPase", J. Exp. Biol 172:93-103.
  16. R.D. Doherty and P.M. Kane, (1993) "Partial assembly of the yeast vacuolar proton-translocating ATPase in mutants lacking one subunit of the enzyme",  J. Biol. Chem. 268: 16845-16851.
  17. P.M. Kane (1995), "Disassembly and reassembly of the yeast vacuolar H+-ATPase in vivo", J. Biol. Chem. 270:17025-17032.
  18. Q. Liu, P.M. Kane,  P. Newman,  and  M. Forgac (1996) "Site-directed mutagenesis of the yeast V-ATPase B subunit", J. Biol. Chem. 271: 2018-2022.
  19. K.J. Parra and P.M. Kane (1996)  "Wild-type and mutant vacuolar membranes support pH-dependent reassembly of the yeast vacuolar H+-ATPase in vitro", J. Biol. Chem. 271: 19592-19598.
  20. J. Liu and P.M. Kane (1996) "Mutational analysis of the catalytic subunit of the yeast vacuolar proton-translocating ATPase", Biochemistry 35: 10938-10948.
  21. Q. Liu, X-H. Leng, P.R. Newman, E. Vasilyeva, P.M. Kane, and M. Forgac (1997) “Site directed mutagenesis of the yeast V-ATPase A subunit”, J. Biol. Chem. 272: 11750-11756.
  22. Y.E. Oluwatosin and P.M. Kane (1997) “Mutations in the CYS4 gene provide evidence for regulation of the yeast vacuolar H+-ATPase by oxidation and reduction in vivo”, J. Biol. Chem. 272:  28149-28157.
  23. Y.E. Oluwatosin and P.M. Kane (1998) “Mutations in the yeast KEX2 gene cause a Vma-like phenotype:  a possible role for the Kex2 endoprotease in vacuolar acidification”, Mol. Cell. Biol. 18:  1534-1543.
  24. J.W. Zhang, K.J. Parra, J. Liu, and P.M. Kane (1998) “Characterization of a temperature-sensitive vacuolar ATPase mutant with defects in actin distribution and bud morphology”, J. Biol. Chem. 273: 18470-18480.
  25. K.J. Parra and P.M. Kane (1998) “Reversible association between the V1 and Vo domains of yeast vacuolar H+-ATPase is an unconventional glucose-induced effect”, Mol. Cell Biol. 18: 7064-7074.
  26. P.M. Kane (1999) “Introduction:  V-ATPases 1992-1998”, J. Bioenerg. Biomemb. 31: 3-5.  (Invited review—Introduction to review volume)
  27. P.M. Kane (1999)  “Biosynthesis and regulation of the yeast vacuolar H+-ATPase”, J. Bioenerg. Biomemb. 31: 49-56.  (Invited review)
  28. P.M. Kane, M. Tarsio, J. Liu (1999)  “Early steps in assembly of the yeast vacuolar H+-ATPase”, J. Biol. Chem. 274: 17275-17283.
  29. N. Hernando, P. David, M. Tarsio, M. Barkiewicz, W.C. Horne, P.M. Kane, R. Baron (1999).  “The presence of the alternatively spliced A2 cassette in the vacuolar H+-ATPase subunit A prevents assembly of the catalytic domain, “  Eur. J. Biochem. 266: 293-301
  30. P.M. Kane and K.J. Parra (2000)  “Assembly and regulation of the yeast vacuolar H+-ATPase”, J. Exp. Biol. 203: 81-87. (Invited review)
  31. P.M. Kane (2000)  “Regulation of V-ATPases by reversible disassembly”, FEBS Lett. 469: 137-141.  (Invited review)
  32. K.J. Parra, K.L. Keenan, and P.M. Kane (2000)  “The H subunit (Vma13p) of the yeast V-ATPase inhibits the ATPase activity of cytosolic V1 complexes”, J. Biol. Chem. 275: 21761-21767.
  33. C.M. Charsky, N.J. Schumann, N.J., and P.M. Kane (2000)  ”Mutational analysis of subunit G (Vma10p) of the yeast vacuolar H+-ATPase” J.  Biol. Chem. 275: 37232-37239.
  34. C. Forster and P.M. Kane (2000)  “Cytosolic Ca2+ homeostasis is a constitutive function of the V-ATPase in Saccharomyces cerevisiae” J. Biol. Chem. 275: 38245-38253.
  35. K.K. Curtis and P.M. Kane (2002) “Novel V-ATPase complexes resulting from overproduction of Vma5p and Vma13p”, J. Biol. Chem. 277:2716-2724.
  36. K.K. Curtis, S.A. Archibold, Y. Oluwatosin, and P.M. Kane (2002)  “Mutational analysis of subunit C (Vma5p) of the yeast vacuolar H+-ATPase”, J. Biol. Chem. 277: 8979-8988.
  37. A.M. Smardon, M. Tarsio, and P.M. Kane (2002) “The RAVE complex is essential for stable assembly of the yeast V-ATPase”, J. Biol. Chem. 277: 13831-13839.
  38. P.M. Kane and A.M. Smardon (2003) “Assembly and regulation of the yeast vacuolar H+-ATPase”, J. Bioenerg. Biomemb. 35(4):313-321. (Invited review)
  39. Z. Zhang, C. Charsky, P.M. Kane, and S. Wilkens (2003) “Yeast V1-ATPase:  Affinity purification and structural features by electron microscopy”, J. Biol. Chem. 278(47): 47299-47306.
  40. A.B. Parsons, R.L. Brost, H. Ding, Z. Li, C. Zhang, B. Sheikh, G.W. Brown, P.M. Kane, T.R. Hughes, and C. Boone (2004) “Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathways”, Nature Biotechnology 22(1): 62-69.
  41. M. Sambade and P.M. Kane (2004), “The yeast V-ATPase contains a subunit homologous to the M. sexta and bovine e subunits that is essential for function”, J. Biol. Chem. 279:17361-17365.
  42. M. Sambade,  M. Alba, A. M. Smardon, R.W. West, and P.M. Kane (2005) “A genomic screen for yeast vma (vacuolar membrane ATPase) mutants”, Genetics, 170(4):1539-1551.
  43. M. Liu, M. Tarsio, C. M. Charsky, and P.M. Kane (2005) “Structural and functional separation of the N- and C-terminal domains of the yeast V-ATPase subunit H”, J. Biol. Chem. 280:36987-36985.
  44. P.M. Kane (2005) “Close-up and genomic views of the yeast vacuolar H+-ATPase”, J. Bioenerg. Biomemb. 37(6): 399-403. (Invited review)
  45. P.M. Kane (2006) “The how, when and where of organelle acidification by the yeast vacuolar H+-ATPase”, Microbiology and Molecular Biol. Reviews, 70 (1):177-191.
  46. M. Ohira, A.M. Smardon, C.M.H. Charsky, J. Liu, M. Tarsio,and P.M. Kane (2006) “The E and G subunits of the yeast V-ATPase interact tightly and are both present at more than one copy per V1  complex”, J. Biol. Chem 281: 22752-22760.
  47. E. Milgrom, H. Diab, F. Middleton, and P.M. Kane (2007) “Loss of V-ATPase activity in yeast results in chronic oxidative stress”, J. Biol. Chem., 282:7125-7136.
  48. J.M. Rizzo, M. Tarsio, and P.M. Kane (2007) “Diploids heterozygous for a vma13∆ mutation in S. cerevisiae highlight the importance of V-ATPase subunit balance in supporting vacuolar acidification and silencing cytosolic V1-ATPase activity”, J. Biol. Chem. 282:8521-8532.
  49. A.M. Smardon and P.M. Kane (2007) “RAVE is essential for the efficient assembly of the C subunit with the vacuolar H+-ATPase”, J. Biol. Chem. 282:26185-26194.
  50. P.M. Kane (2007) “The long physiological reach of the yeast vacuolar H+-ATPase”, J. Bioenerg. Biomemb. 39: 415-421.
  51. G. A. Martinez-Munoz and P.M. Kane (2008) "Vacuolar and plasma membrane proton pumps collaborate to achieve cytosolic pH homeostasis in yeast", J. Biol. Chem., 283:20309-203019.
  52. S. Chen, M. Tarsio, P.M. Kane, and M.L. Greenberg (2008) "Cardiolipin mediates cross talk between mitochondria and the vacuole", Mol. Biol. Cell 19:5047-5058.
  53.           Z. Zhang, Y. Zheng, H. Mazon, E. Milgrom, N. Kitagawa, E. Kish-Trier, A.J. Heck, P.M. Kane, and S. Wilkens (2008) "Structure of the yeast vacuolar ATPase", J. Biol. Chem., 283:35983-35995.
  54. S.C. Li and P.M. Kane (2009) "The yeast lysosome-like vacuole:  Endpoint and crossroads", Biochim. Biophys. Acta,, 1793:650-663.
  55. H. Diab, M. Ohira, M. Liu, E. Cobb, and P.M. Kane (2009) "Subunit interactions and requirements for inhibition of the yeast V1-ATPase", J. Biol. Chem. 284:13316-13325.
  56. T. Diakov and P.M. Kane (2010) “Regulation of V-ATPase activity and assembly by extracellular pH”, J Biol. Chem. 285:23771-23778.
  57. M. Tarsio, H. Zheng, A.M. Smardon, G.A. Martinez Munoz, P.M. Kane (2011) "Consequences of loss of Vph1-containing V-ATPases for overall cellular pH homeostasis", J. Biol. Chem. 286:28089-28096.
  58. P.M. Kane (2012) "Targeting reversible disassembly as a mechanism of controlling V-ATPase activity", Current Protein and Peptide Science 13:117-123.
  59. S.C. Li, T. T. Diakov, J.M. Rizzo, and P.M. Kane (2012) "The V-ATPase works in parallel with the HOG pathway to adapt yeast cells to osmotic stress", Eukaryotic Cell 11:282-291.
  60. M. Lin, S.C. Li, P.M. Kane, and T. Hofken (2012) "Regulation of vacuolar H+-ATPase activity by the Cdc42 effector Ste20 in Saccharomyces cerevisiae," Eukaryotic Cell 11:442-451.
  61. H. I. Diab and P.M. Kane (2013) "Loss of vacuolar H+-ATPase (V-ATPase) activity in yeast generates an iron deprivation signal that is moderated by induction of the peroxiredoxin TSA2 (2013) J. Biol. Chem. 288:11366-11377.
  62. T.T. Diakov, M. Tarsio, P.M. Kane (2013) "Measurement of vacuolar and cytosolic pH in vivo in yeast cell suspensions", J. Vis. Exp. Apr 19;(74). doi: 10.3791/50261.            
  63. A.M. Smardon and P.M. Kane (2014) "Vacuolar H+-ATPase assembly" in Handbook of ATPases, S. Nakamura Ed., Pan Stanford Publishing, Singapore.
  64. A.M. Smardon, H.I. Diab, M. Tarsio, T.T. Diakov, R.W. West, and P.M. Kane (2014) "The RAVE complex is an isoform-specific V-ATPase assembly factor in yeast" Mol. Biol. of the Cell 25(3): 356-367.
  65. S.C. Li,  T.T. Diakov, T. Xu, M. Tarsio, S. Couoh-Cardel, L.S. Weisman, and P.M. Kane (2014) "The signaling lipid PI(3,5)P2 stabilizes V1-Vo sector interactions and activates the vacuolar H+-translocating ATPase" Mol. Biol. of the Cell 25(8):1251-1262
  66. A.M. Smardon and P.M. Kane (2014) "Loss of vacuolar H+-ATPase activity in organelles signals ubiquitination and endocytosis of the yeast plasma membrane proton pump Pma1" J. Biol. Chem.289(4): 32316-26.
  67. R.M. Deranieh, Y. Shi, M. Tarsio, Y. Chen, J.M. McCaffery, P.M. Kane, and M.L. Greenberg (2015) "Perturbation of the vacuolar-ATPase:  A novel consequence of inositol depletion", J. Biol. Chem. 290:27460-27472.
  68. A.M. Smardon, N.Dehdar Nasab, M. Tarsio, T.T. Diakov, and P.M. Kane (2015) "Molecular interactions and cellular itinerary of the yeast RAVE (Regulator of the H+-ATPase of vacuolar and endosomal membranes) complex",  J. Biol. Chem. 290:27511-27523.
  69. P.M. Kane (2016) "Proton transport and pH control in fungi", in Yeast Membrane Transport, Ed. by J. Ramos et al., Advances in Experimental Medicine and Biology 892, 33-68.
  70. R. Oot, P.M. Kane, E.A. Berry, and S. Wilkens (2016) "Crystal structure of yeast V1-ATPase in the autoinhibited state", EMBO J. 35:1694-1706.
  71. S. Banerjee and P.M. Kane (2017) “Direct interaction of the Golgi V-ATPase a-subunit isoform with PI(4)P drive localization of Golgi V-ATPases in yeast”, Mol. Biol. Cell 28:2518-2530.
  72. S.D. Velivela and P.M. Kane (2018) “Compensatory internalization of Pma1 in V-ATPase mutants in S. cerevisiae requires calcium- and glucose-sensitive phosphatases”, Genetics 208:655-672.
  73. L.A. Graham, G.C. Finnigan, and P.M. Kane (2018) “Some assembly required: Contributions of Tom Stevens’ lab to the V-ATPase field”, Traffic 19:385-390.  (invited review)
  74. P.M. Kane (2018) “Energy powerhouses of cells come into focus” Science 360:600-601 (invited perspective).
  75. S. Banerjee and P.M. Kane (2018) “Isoform-specific activation of yeast V-ATPase complexes by two phosphatidylinositol phosphate lipids”, submitted.

Current

2018-2022 National Institutes of Health  R01 GM127364 , “Mechanisms of RAVE-induced V-ATPase assembly”, Total costs $1.25 million (Kane, P.I.)

2018-2022 National Institutes of Health  R01 GM126020, “Regulation of V-ATPases by phosphoinositides”, Total costs $1.1 million (Kane, P.I.)

 

Previous

1994-2018 National Institutes of Health (1 R01 GM50322), "Subunit Structure and Function in Vacuolar H+-ATPases" (Kane, P.I.)

2002-2007 National Institutes of Health (1 R01 GM63742), “A Skp1-containing Complex Regulating ATPase Activity” (Kane, P.I.)

1996-2001 American Heart Association, Established Investigatorship

1993-1996 American Heart Association, New York State Affiliate, "Subunit Structure and                Function in the Yeast Vacuolar H+-ATPase"

1991-1996 National Science Foundation, 1991 Presidential Young Investigator Award,     

1992-1993 SUNY Hendricks Fund Equipment Grant

1991-1993 American Chemical Society, PRF Type G Award, “Coupling of ATP Hydrolysis and Proton Transport by the Yeast Vacuolar Proton-translocating ATPase”

1991-1992 Bristol-Myers Squibb Company Grant of Research Corporation

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